ISSX Workshop on Quantitative LC-MS/MS Proteomics: Methodology and Applications in Translational DMPK/PD and Precision Medicine
An ISSX In-Person Workshop | June 27-28, 2024 | Certara, Sheffield, United Kingdom
Workshop Background
In September 2018, ISSX held its very successful workshop on quantitative LC-MS proteomics as applied to drug development. The event was addressing a niche but growing utility area for LC-MS proteomics. Hence it involved small gathering of experts who debated unresolved issues and tried to provide a consensus on various elements of using quantitative LC-MS proteomics in translational DMPK/PD research.
The consensus, as well as issues which were not settled, appeared in an article that was published in Clinical Pharmacology and Therapeutics in 2019 (Prasad et al.). Since then, many groups have started to use LC-MS proteomics techniques and published on applications of this methodology. These reports have encouraged many other labs to consider getting engaged with this approach and integrate it into their research capabilities. However, there is currently no dedicated workshop or a course that takes the interested individual through the process of “setting Up an LC-MS lab for quantitative proteomics, conduct of experiments, analysis of raw data coming out of machine read out, and interpreting and applying them in translational pharmacology (whether PK or PD). This is addressed by the current workshop.
Learning Objectives
The workshop is neither trying to provide a 101 course on LC-MS proteomics nor attempts to focus on what is new in LC-MS proteomics techniques. Attendees need to have some background but not necessarily leading experts in the field. The consensus report from the previous ISSX Workshop held in 2018 (Prasad et al. 2019, Clinical Pharmacology and Therapeutics) is considered a background reading. The recent Symposium Repot from North American ISSX 2023 in Boston (Prasad et al 2024, Drug Metabolism Disposition, In Press) captures the content of lectures and workshop exercises. Hence, the event builds on what is known in the literature in relation to LC-MS proteomics and provides more practical guidance for those who are expanding their research activities in this area, such that they do not go through pitfalls and get to the end point faster.
Session 1 – Become familiar with various proteomics techniques, necessary instrumentation, and protocols. It also provides some advice on cost-benefit ratio of various alternatives based on experience of group of individuals who have tried such alternatives.
Session 2 – Understand specific sample preparation techniques and the rationale in employing such techniques regarding each matrix (various tissues, cell lines). The common pitfalls are described and the impact on end result are explained with a take-home list of do’s and don’ts.
Session 3 – Recognize the steps for data analysis applied to raw data from LC-MS machine and associate them with the appropriate software that assist with such analysis. The latter would be through detailed examples lead by tutors from the front.
Session 4 – Enable the participants to widen their knowledge of applications beyond what they might have known and indicate examples of ongoing research which benefit from incorporation of LC-MS proteomic data in relation to drug development cases.
